Journal: Immunity

Article Title: Identification of a Kupffer cell subset capable of reverting the T cell dysfunction induced by hepatocellular priming

doi: 10.1016/j.immuni.2021.05.005

Figure Lengend Snippet:

Article Snippet: Primary Abs include anti-STAT5 and anti-pSTAT5 (Tyr694) (rabbit; Cell Signaling Technology #8215) and β-actin (polyclonal; Abcam ab228001).

Techniques: Purification, Recombinant, Liposomes, Fluorsave, Staining, Cell Isolation, DNA Library Preparation, Isolation, RNA HS Assay, Western Blot, Gene Expression, Software, Microscopy

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 9 Expression of HK2 and STAT5 mRNA in the bovine mammary gland. Expression levels of HK2 and STAT5 in lactating and non-lactating bovine mammary glands were determined using real-time PCR. Gene fold changes were calculated using the 2−ΔCt

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 8 Putative miRNAs targeting gene 3’UTRs. (A) Pairing of bta-miR-141 with the bovine STAT5 3’UTR sequence. (B) A target seed region of the miRNA binding site was found within the bovine HK2 3’UTR sequence.

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Sequencing, Binding Assay

Journal: BMC genomics

Article Title: Expression profiles of microRNAs from lactating and non-lactating bovine mammary glands and identification of miRNA related to lactation.

doi: 10.1186/1471-2164-13-731

Figure Lengend Snippet: Figure 10 STAT5 protein is regulated by miR-141. STAT5 and β-actin protein expression was determined by western blotting. Mac-T cells were transfected with an miR-141 inhibitor, an miR-141 mimic, an inhibitor negative control (INC) and a negative control (NC). Cell lysates were collected after 24 hours and analyzed by western blotting. Beta-actin served as the internal control. The protein fragment intensity was quantified using Imagpro-Plus software, and IOD was used to represent the protein level. Error bars represent SD. n=3. a-c means different superscripts differ (P<0.05).

Article Snippet: Antibodies for STAT5 and β-actin were manufactured by Boster (China), and the HK2 antibody was purchased from Santa Cruz (USA).

Techniques: Expressing, Western Blot, Transfection, Negative Control, Control, Software

Journal: Cell reports

Article Title: Activation of Mevalonate Pathway via LKB1 Is Essential for Stability of T reg Cells.

doi: 10.1016/j.celrep.2019.05.020

Figure Lengend Snippet: Figure 7. GGPP Enhances STAT5 Activation to Maintain Treg Lineage Stability (A) Purified naive CD4 T cells were differentiated for Th1, Th17, and Treg differentiation conditions with or without GGPP. Percentages of in vitro differentiated Th1, Th17, and Treg cells were shown. Data shown are mean ± SEM. (B) Protein prenylation state in untreated Treg cells and Treg cells treated with geranylgeranyltransferase I inhibitor (GGTi; 10 mM) or GGPP (10 mM) sorted from 3-week-old WT and LKB1fl/flFoxp3-Cre mice. (C and D) Purified Treg cells from 3-week-old WT and LKB1fl/flFoxp3-Cre mice were isolated and stimulated with anti-CD3/CD28 with or without GGPP (10 mM) for 24 h. (C) Immunoblot analysis of STAT5 activation in resting and IL-2-stimulated Treg cells is shown. (D) Immunoblot analysis of Smad2/3 activation in resting and TGF-b-stimulated Treg cells is shown. b-actin serves as a loading control. Results represent three independent experiments. *p < 0.05.

Article Snippet: Cell lysates were fractionated by SDS-PAGE and analyzed by immunoblot with antibodies to p-LKB1, LKB1, p-AMPK, p-ACC, ACC, p-mTOR, mTOR, Bcl-xL, pSTAT5, STAT5, pSMAD2/3, Smad2/3 were from Cell signaling; HMGCR and SREBP-1 were from Santa Cruz Biotechnology; AMPK, Mark and Foxp3 were from Abcam; HMGCS1 was from Biorbyt. b-catenin was from Sigma-Aldrich.

Techniques: Activation Assay, In Vitro, Isolation, Western Blot, Control

Journal: Journal of Veterinary Internal Medicine

Article Title: Histopathologic, phenotypic, and molecular criteria to discriminate low‐grade intestinal T‐cell lymphoma in cats from lymphoplasmacytic enteritis

doi: 10.1111/jvim.16231

Figure Lengend Snippet: Pattern A shows low‐grade intestinal T‐cell lymphoma (LGITL) case with CD20 aberrant expression and differential expression of negative pSTAT3 and positive pSTAT5. At higher magnification (×400), intraepithelial and lamina propria lymphocytes display the same phenotype. Pattern B shows LGITL case with an apical‐to‐basal infiltration (CD3+ partial infiltration of the villi and superficial lamina propria) and a lymphoplasmacytic enteritis (LPE) case. Differential expression of pSTAT3 and pSTAT5 between LGITL and LPE may help to discriminate both diagnoses: tumoral T‐cells are pSTAT3− and pSTAT5+ whereas reactive inflammatory cells are pSTAT3+ and pSTAT5 mostly negative

Article Snippet: The STAT3/STAT5 pathway was evaluated using pSTAT3 Y705 antibodies (Phospho‐STAT3; 1:100 dilution, D3A7, Cell Signaling, Danvers, Massachusetts) and pSTAT5 Y694/699 antibodies (Phospho‐STAT5; 1:100 dilution, polyclonal antibody, Biorbyt, Cambridge, United Kingdom).

Techniques: Expressing, Quantitative Proteomics